How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can anyone help me to separate my phenolic compound in TLC with any new solvent system ?

My Compound is Leaf powdered Methanolic extract which is showing good phenolic compounds upon dipping in FeCl3. -My problem is phenolic compounds are not eluting with any common solvent system.

22 January 2021 4,693 7 View

Suggest best performance evaluation metrics for image segmentation?

Good morning everyone. As a part of my research work, I designed a network that extracts the leaf region from the real field images. when I search about performance evaluation metrics for the...

10 January 2021 9,581 30 View

Easy and effective method to visualize fungal mycelium inside leaf tissue?

Is there any easy and efficient method to visualize the fungal mycelium inside infected leaf tissue using light microscoopy? Please share protocols which can be performed using limited resources...

03 January 2021 4,217 2 View

What is the scope of ETc within the domain of precision irrigation?

Within the domain of precision irrigation, ETc is the daily amount of moisture lost due to evaporation and transpiration. This amounts to the crop's daily water need, as I understand things. Is...

21 December 2020 5,662 4 View

How can we find crop transpiration (Tr) value from ETo values in Aquacrop model?

it like how we can get Tr value separately from ETo values in Aquacrop

20 December 2020 5,817 3 View

Can someone help me find a way to quantify leaf shape?

I 'am looking for a most simplified formula to quantify shape of leaves mathematically which will help me to categorise plants based on leaf shape.Earlier,I tried with some image processing...

10 December 2020 2,386 5 View

Why does silver nitrate turn brown upon addition of leaf extract but no increase in absorption at 400 - 500 nm?

I am currently biosynthesizing silver nanoparticles using ethanolic leaf extract. Upon addition of the leaf extract to 1mM silver nitrate, the solution turns brown in color after exposure to...

08 November 2020 1,009 6 View

Can you suggest the encapsulation method for leaf part of the plant?

Greetings, I am interested to study the effects of encapsulated leaf extracts on the self-healing property of a coating. Can anyone suggest a suitable method for me to fabricate this micro OR...

05 November 2020 327 4 View

ELISA against plant viruses?

We are doing DAC ELISA against plant viruses. I have following queries. Controls that we are using are as follows. Buffer control, Healthy leaf samples, disease infected sample (+ve sample), and...

14 October 2020 1,627 2 View

Which one is best for extraction method MICROWAVE OR Soxhlet for leaf components?

I want to isolate components from leaf material, so I have beter solvets but suggest me method which will improve yield and purity

08 September 2020 1,003 2 View