I am planning to test some plant extracts for their efficacy as hypoglycaemic agents. I am reluctant to use even a lower dose of streptozotocin to induce diabetes in rodents. If anybody have experience in inducing diabetes may be through dietary means within a short duration, could you please let me know how long it will normally take and what rodent strain is most suitable.
You can go for High fat diet and Fructose/sucrose to induce diabetes and insulin resistant models.. it would take for 40 days to 60 days... High fat diet may include cholesterol, cholic acid like that.. fructose or sucrose can be dissolved in water and given instead ordinary water..
If you are talking about Type 2 diabetes, and don't want to use STZ, then I would recommend ZDF fa/fa rats for your research. Good luck...
I would advise against using STZ if T2D is your interest. Use a leptin receptor deficient strain of rat like the ZDF fa/ fa or ob/ob mouse. Make sure you are feeding the correct diet when using these models. The male ZDF must be fed 5008 diet (16% fat), but the females need a very high fat diet (48%) to become hyperglycemic.
We use in our lab the db/db mouse, and also TallyHo as a strain the develop Type 2 diabetes on a polygenetic background. TallyHo on a high-fed diet will become diabetic with an age of around 2 month. For lean mouse models we use C3H on high-fed diet (saff-flower-oil) but this will take longer till they get diabetic.
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