One of the best method for storing fungal species using cryotubes with Glycerol 30% at -80ºC for longer storage or -20ºC for continuous use.

Thank you, we have been using a 15% glycerol solution.

To improve the preservation performances it's better to leave the cryotubes containing the fungal strain under glycerol at least for one hour at room temperature, then proceed with a gradual freezing. In this regard I suggest you freezing boxes such as those reported at:

http://www.thermoscientific.com/ecomm/servlet/productsdetail?storeId=11152&productId=11964137&ca=mrfrosty

For yeasts lyophilization is widely used.

thank you Daniela

what about other (not only yeasts) - filamentous fungi for long-term storage? Do you cultivate them in liquid media and then add glycerol (mixed) and freeze?