hi Bro!!

did u try using combination of penstrep (penicillin and streptomycin) while culturing??

or you can pass the RBC suspension before culturing, through a 40 micron cell strainer, to get rid of fungal contaminations and other debris that lead to contaminations while culturing.

Hello! As far as I know, all existing sterilization methods would bring considerable biochemical, etc. changes into your blood sample, so the best option is probably to take a fresh blood sample at sterile conditions into a sterile container. If few bacteria occur in your sample, they are usually killed/inactivated by intrinsic blood brotection systems but only if blood is fresh.

Yup!! It absolutely depends on your aim of using blood cell culturing. Usage of antibiotics may lead to recovery and infection free system in which you may not be able to study any disease related issues. So, first would be to maintain basic sterility, for eg: sterile syringes, vaccutainers, sterile collection tubes, sterile culture facility.separate incubation for blood samples etc..

You can use photodynamic therapy.

Thanks dear Sabyasachi , Ilya and Almeida for your valuable suggestion .

I can not use antibiotics because I want to use this sterilize blood for the growth of particular microbes.

Dear, at first I agree with Sabyasachi Dash. After trying that, if you really need sterilization treatment, clearly it will depend on what you want to do. If you don't need intact structure you can perform tangential filtration using a 0.2 µm column (or 0.1µm) after a cell lysis (for example using microbead shaking) and a first centrifugation cycle. You will lost a part of your compound but keep significant amount of protein, fats... You can also use ionizing radiation for small amount to treat.

I am using photodynamic therapy to sterilize blood and I have reductions of 6 log for Gram negative bacteria. After treatment I do not use the blood, the samples go to waste but could be used for other tests, like yours.

Are you trying to keep the blood components intact? If so, you can follow the practices of the blood banking industry. In the blood banking industry, it is standard practice to operate as much as possible within closed containers. Once your blood product has been collected from the source as aseptically as possible, there are a variety of methods you can use for manipulation and fluid addition, such as sterile-docking/tube-welding or using aseptic technique with injection sites. These two technologies usually help to reduce the opportunity for contamination.