What should I do for my controls not to stain my secondary antibody ( IHC for mice muscle) ?

I am staining mice muscle for macrophages (CD68) and lymphocytes (CD3). The challenge that am facing is that my control is also staining with the secondary antibody even though I did not add the...

15 October 2020 8,139 3 View

Triangulation of data sources in qualitative research: Do you consider it mixed methods research and why, whether its a yes or no?

It is common in qualitative research to interview for example patients and their partners or their health professionals to talk about the same topic and explore the perspectives of all the people...

30 July 2020 538 21 View

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house ?

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house that is a step down from inpatient unit. The study will look at...

12 May 2020 7,072 3 View

Who can link me to Cost / Benefit Analysis of Treatment Options for Produced Water of varying quality and quantity?

I work in the oil industry and I wish to focus on reducing reinjection of "free" (non-water right controlled) produced water for beneficial use where economics dictate feasibility. Who can direct...

24 February 2020 3,059 2 View

How is confirmatory factor analysis not just a tautology?

Hello, very new to confirmatory factor analysis (CFA) and structural equation modeling, and I'm trying to understand how to think about confirmatory factor analysis. Suppose I have a construct,...

11 November 2019 3,330 4 View

How to deal with member check conflicts in qualitative research?

Member checking or participant checking is done to validate and improve the credibility of quallitative research results. I am planning to ask my participants to help with that (will ask them to...

27 May 2019 7,799 13 View

How do you create a calibration curve for H2O2 ?

Specifically I'll be looking at H2O2-titanium complexes (in plant tissue) and will be measured @ 415nm. I'm wondering how I can create a calibration curve for the complex... I have 30% H2O2 and...

13 December 2018 4,432 3 View

Improvised abacus? Are there Latin references to betting at Roman amphitheatres, or modern references to Roman use of abaci? Or was it a gaming board?

11 June 2015 1,010 68 View

Do we have instrument development experts among rehabilitation professionals in Sub-Saharan Africa?

It has been noted that clinicians in Africa are forced to use instrument measures that were developed and validated either for American and European children which are quite limited. It is...

01 January 1970 528 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View