DGGE or Clone Library Construction, taking into account a more simple method to perform, reproducibility, cost, time, efficiency.
I suggest the NGS to determinate microbial diversity. Thought it is a little expensive, it is efficient and more accurate than DGGE or Clone Library Construction
I guess 16s rRNA targeted NGS is better, although it is bit costly but its resolution is better than other two methods.
DGGE is difficult, CLC is laborious and actually expensive. NGS by pyrosequencing method is most popular but include bias. Read whole metagenome and sorting rRNA gene sequence is bias free but require extensive informatic works. I thingk, NGS by pyrosequencing is still best in accuracy, price, time, etc. * chunlab.com
I suggest NGS (Ion torrent PGM platform) is better for studying microbial diversity.
I also suggest NGS.
For microbial community analysis and functional assays, Biolog Ecoplates. Cheap, quick and easy procedure.
Thanks to all, but between two techniques DGGE and Library what is the best?
There is no single answer to your query.
To study the change in the structure of microbial communities, DGGE is the best. But if the diversity is too high, it would fail.
If you consider the simplicity and time, Clone library construction is the best.
But in both the methods, reproducibility depends on the user's expertise.
Clone library construction is cheaper whereas DGGE equipment is costly.
Above the experts advice is right. NGS is better than CLC and DGGE.
Based on you have to choose from these two methods, so:
CLC is more accurate than DGGE, if microbiology diversity is high. But CLC is laborious.
You could apply them together.
First, you can see the whole change by DGGE, if you'd like to see more accurate differences, then, you could apply CLC after filtering DGGE results. This could reduce clone libraries you need to construct.
How I can to determinate the morphological and molecular protozoa riches in water samples (rivers)
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