We are working on project on Determination of potential of soil bacteria to biodegrade pesticide residues along lake Victoria basin and also determine the genetic diversities of these bacteria in the region.
hello simon.. you can use COXI or 16SrRNA gene sequence if you want to make benefit og gene bank data to draw phylogeny... or you can use RAPD method if you want simple, easy inexpensive method. i hope you do it well.
The diversity that resides within living organisms, often referred to as biodiversity, is essential for sustained existence and provides the raw materials for agriculture, biotechnology and medicine. Both its conservation and utilization require knowledge of existing variation. Molecular tools provide valuable data on diversity through their ability to detect variation at the DNA level. Although this has many important applications, the overview presented here focuses on the use of molecular techniques for detecting genetic differences, distinguishing between individuals and for identification.
Dear Simon,
The use of molecular techniques is not all; for succes in research it need to have the perfect idea.
In biodégradation it need to study the conditions of bactérie life !!!
Best regards
Hi Simon.
In order to answer this question in a helpful manner, I would suggest that you provide a little more context. Most geneticists would agree that the best way to address genetic diversity generically speaking, is to do whole genome sequencing, but this approach is likely more expensive and more involved than the capabilities and access to equipment would allow for many researchers.
But appropriate answers to your question will require you to provide what organism you are interested in, as specific markers can be more or less informative depending on the lineage in question (i.e. plant, fungus, animal, vertebrate, invertebrate, prokaryote, etc. I think you get the idea).
In addition number of samples to be processed, and finally whether you have access to and budget for molecular approaches such as PCR, agarose gel electrophoresis, DNA sequencing, etc.
It looks like you're interested in bacteria, there have been many informative method papers, including review papers, published on this topic, a sampling of markers used includes:
16S rRNA, 23S rRNA, rpoB, gyrB, dnaK, dsrAB, amoA, amoB, mip, horA, hitAM, recA, ica, frc. oxc, 16S-23S rDNA ISR and IS256.
Mol Gen Mikrobiol Virusol. 2012: (3):14-7. Popular molecular markers in bacteria. Liu W1, Li L, Khan MA, Zhu F.
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