In the laboratory we have used an antioxidant analyzer with an amperometric detector and DPPH. We now need new techniques to compare.
Try spectrophotometer based dpph and abts free radical scavenging assays for estimation of antioxidants especially phenolics
but if sample contains lyophilic antioxidants with lower polarity than you can use dpph assay estimated by electron paramagnetic resonance (epr) following
optimization of the extraction of antioxidative constituents of six barley cultivars and their antioxidant properties. J. Agric. Food chem. 2006, 54, 8048–8057.
Some time ago I was at a seminar were a new device (at least new to me) for the measure of antioxidant capacity based on chemiluminiscence. PHOTOCHEM.
It was very interesting and the technicians told me that they can analyze samples I sent them to test the possibilities of the machine.
Take a look at the web page and aplications if you don´t know them:
http://www.analytik-jena.de/en/analytical-instrumentation/products/antioxidants.html
Indeed, none of the above methods measures bioavailability of any antioxidant. You could estimate the impact of food antioxidant on the organism antioxidative defence system only. If you want to quantify bioavailability of some antioxidant you actually need to use compound-specific pharmacokinetic protocol(s).
- Badges
- Science topic