What is the best method of removing high abundance proteins for proteomic analyses?

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Can I use DNase I and RNase R in the same moment?

Hi, I want to obtain circular RNA and lariat. I need treat my samples with DNase I and RNase R. Do you know, I can do this in the same time? And maybe do you have any protocols for simultaneous...

02 June 2024 9,544 0 View

What are the possible effects of DTT or β-mercaptoethanol, on gDNA amplification?

I used the Intact Genomics FastAmp® Plant Direct PCR kit and when seeding the gel with the PCR product there was a lot of DTT smell. After running the gel, partially degraded dna was observed,...

10 April 2024 2,150 2 View

How can we accurately determine the lactate threshold when there is a delay in the appearance of lactate in the blood?

I am well aware of the methodology for determining lactate threshold; that is not my question. My doubts concern the physiology of lactate transportation to the blood. Once lactate is formed in...

26 February 2024 4,579 0 View

Is there a way to measure firefly and renilla luciferase luminescence in organoids grown in matrigel?

Hi, I am interested in performing a reporter assay in organoids grown in matrigel. Is there a commercially available kit that would allow me to read both firefly and renilla luciferase...

06 February 2024 6,029 0 View

It's possible to know the weights that the package deaR (in R program) assigns to each input?

Hi! I want to ask if is there any way to know the weights that the package assigns to each input to make the analysis. If is there any way that would be awesome to my research of forest Regards!

06 November 2023 4,316 0 View

Hi! Does anyone know of references using strip transect data for habitat selection analysis, or more specifically resource selection function (RSF)??

I have GPS locations of animals in which I will use for a resource function analysis. However, this data follows the transects from aerial transect counts and are aligned on lines. I therefore...

19 October 2023 8,438 1 View

Why do I get an arc when I electroporate Agrobacterium?

I prepared electrocompetents of the AGL1 strain following the "water" protocol: the bacterial culture was resuspended in water, subjected to two washes in water, one in 10% glycerol, and finally...

17 October 2023 4,770 3 View

What kind of test tubes do you use to prepare bacterial suspensions in biomerieux densitometers?

Hi, We are currently using densitometers from biomerieux to prepare bacterial suspensions. We are wondering if there are any tubes with sterile NaCl solution available on the market other than...

25 September 2023 9,673 4 View

What R program or python program do you recommend for analyzing RNA for secondary structure and finding sequential-structural motifs?

Hi! I'm using an R program and I have a large table where I have UTR sequences. I would like to parse these sequences from within R (or python) for secondary structure prediction and finding...

08 March 2023 1,835 2 View

What is recommended set-up for laboratorty steam distillation at a reduced pressure?

Most textbooks provide details about either steam or vacuum distillation. I am looking for a description of the apparatus for distilling with saturated steam at reduced pressure. Do you have any...

13 February 2023 9,276 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

Can anyone kindly suggest research topics in the area of thermodynamics, heat transfer, fluid mechanics or renewable energy?

I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....

24 July 2024 4,058 5 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Contaminant cells in pancreatic cancer patient derived organoids?

Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...

15 July 2024 762 3 View

I came across oscillations in a pressure profile for a pipeline flow along the axis of a cylinder, how do I prove that these are not numerical err?

In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?

13 July 2024 6,296 5 View

Metallic system in quantum espresso with no smearing. Is this possible?

Hi! I'm simulating a specific material on Quantum Espresso, and I found something strange. Initially I've performed simulations on FCC InAs crystal and I used the Hubbard correction to reproduce...

02 July 2024 9,323 1 View

How to solve the question of ansys fluent?

Regarding the system coupling issue where the update of the solution component fails, the system coupling update for fluid flow (Fluent) triggered an exception: "Update Solution Failed."

30 June 2024 7,894 1 View

How closely does an elastic sheet conform to what's below it?

See the attached file for an illustration of this problem. Consider an elastic sheet S (orange) that’s laying atop a solid base B (blue). The sheet is under tension (arrows) and is held to the...

30 June 2024 747 4 View

Markus Sack

For human plasma there are dedicated products for your case.

Look e.g. at Pierce™ Top 12 Abundant Protein Depletion Spin Columns

Cheers Markus

Emilia Stec-Martyna

Thank you very much for your help.

Camillo Rosano

Ciao Mila!

Depletion columns! Pierce Top12 (as Markus previously suggested) but also Agilent Hu-14 are good choices.

Take care!

Cam