Emilia Stec-Martyna

5 Questions 6 Answers 0 Followers

Questions related from Emilia Stec-Martyna

What is a problem with global methylation test (ALU and LINE) - qMSP method - lack of repeatability and Ct for ACTB greater than 30?

I performed a global methylation test (ALU and LINE) with qMSP method, using TaqMan and TaqMan Universal Master mix probes (reference gene ACTB) in buffy coats. The conversion was performed using...

25 March 2025 5,521 2 View

What is the best method of removing high abundance proteins for proteomic analyses?

I am looking for the best method of removing high abundance proteins (IgG, albumin) for proteomic analyses. I am going to study proteomic pattern of pancreatic cyst fluids by LC-MS/MS.

20 June 2018 4,561 3 View

What is the best protocol of histones isolation from bone marrow?

I am planning to analyze post-translational modifications, including acetylation and methylation of human histones, H3 and H4, via mass spectrometry (LC-ESI-Q-ToF). Can I ask for some advice and...

30 June 2014 8,826 2 View

Does anyone know about methods of histones isolation?

Hi, Does anyone know the method, protocol of histones H3, H4 isolation from the bone marrow? I am specially interested in the method which would be compatible with mass spectrometry (ESI-ToF...

30 June 2014 7,639 2 View

How big can I expect the peptides (what m/z range) to be when using ESI-LC-MS analysis after trypsin digestion?

Generally, I obtain something between 700-2800 Da. How can I get smaller fragments? Is it just the type of enzyme we use?

21 March 2014 6,587 3 View