Emilia Stec-Martyna

3 Questions 4 Answers 0 Followers

Questions related from Emilia Stec-Martyna

What is the best protocol of histones isolation from bone marrow?

I am planning to analyze post-translational modifications, including acetylation and methylation of human histones, H3 and H4, via mass spectrometry (LC-ESI-Q-ToF). Can I ask for some advice and...

30 June 2014 8,742 2 View

Does anyone know about methods of histones isolation?

Hi, Does anyone know the method, protocol of histones H3, H4 isolation from the bone marrow? I am specially interested in the method which would be compatible with mass spectrometry (ESI-ToF...

30 June 2014 7,587 2 View

How big can I expect the peptides (what m/z range) to be when using ESI-LC-MS analysis after trypsin digestion?

Generally, I obtain something between 700-2800 Da. How can I get smaller fragments? Is it just the type of enzyme we use?

21 March 2014 6,540 3 View