I would like to know the best method to determine soil enzymes from a particular soil
These are the methods, I am using for a long time and found most reliable
1. Urease activity : Douglas & Bremner (1970)
2. Lipase-Esterase activity : Margesin et al (2002)
3. Esterase activity : Schnurer and Rosswell (1982)
4. Phosphatase activity : Tabatabai and Bremner (1969)
5. Dehydrogenase activity: ( Casida et al. 1964)
6. Arylsulfatase activity: Tabatabai and Bremner (1970)
7. Beta-Glycosidase activity : Jermyn, (1958) for fungi, Barnett et al. (1956) for yeast and Veibel (1950) for plants
8. Phytase activity : Asmar (1997), Tarafdar et al. (2001)
Dear Dr, Kafula, you can see the attached PDF. It will helpful for you
Thanks Malu
Dear Sir,
pl see these articles.
https://link.springer.com/article/10.1007/BF02181348
http://soilquality.org/indicators/soil_enzymes.html
Kundan Meshram thank you!
Dear Kafula:
You may see also the methodology as in the following ref.:
Tabatabai, M.A. 1994. Soil enzymes. In: Weaver, R.W.,
Angle, J.S., Bottomley PS (eds) Methods of Soil
Analysis, Part 2. Microbiological and biochemical properties.
SSSA Book Series No. 5. Soil Sci. Soc. Am.
Madison, Wis. 775-833.
I agree with J. C. Tarafdar .
Thanks for J. C. Tarafdar recommendation.
Tarafdar and Mandal thanks for the recommendation.
important topic
Some difficulties need to be overcome
soils contain many components (complexes) do interfere with ,,,, the
enzymes&substrates
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