I'm looking for the best method to test in vitro the ability of LAB strains to survive stresses encountered during the passage through the gastrointestinal tract. A test that simulates in vitro the entire GI transit (mouth, stomach, intestines).
Hi Elena,
Stomach acid and bile are the two main obstacles for microbial GI transit. See London et al., J of App Micro (Characterization of a bovine isolate Lactobacillus mucosae DPC 6426 which produces an exopolysaccharide composed predominantly of mannose residues) on my profile for these assays.
If you have a large bank of microbes to screen then it might be useful to PCR for bile salt hydrolase (BSH) genes which would confer protection from primary bile salts. Then follow on with some BSH plate assays.
Hope that helps!
More media you can use for LAB. EXAMPLE: MRS+1.5 bile salt, and add some antibiotic in MRS
Hi Paul and Alaa,
thanks for yours suggestions.
Perhaps I was not enough clear, I know the media to test the stresses to bile salts and acidity. See Succi et al. Bile salt and acid tolerance of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese on my profile.
I wish that you suggest me the best method to simulate in vitro the entire GI transit (mouth, stomach, intestines) with nutrient media, times and stresses.
Article Bile salt and acid tolerance of Lactobacillus rhamnosus stra...
Ahh, apologies. My only suggestion is something like the in vitro simulated digestion protocol attached. This method has been adapted for food faecal fermentation in our lab, but it may be suitable for your work too...
- Badges
- Science topic
- Similar topics
- Computer Communications (Networks)
- Simulators