Samples of leafy vegetables were taken.
Trying to find a proper method to isolate PAHs content of vegetables.
I would appreciate any help
Over the past decades, a variety of techniques such as Soxhlet extraction (SE), ultrasonic extraction (UE), accelerated solvent extraction (ASE), microwave assisted extraction (MAE) and supercritical fluid extraction (SFE), had been widely developed to extract PAHs in environmental ecosystems. In fact, any methods you choose is ok only if you can get good results within strict quality control procedures, such as method detection limits and laboratory method blanks of each target, laboratory fortified blank, recoveries of targets in fortified samples and duplicate extraction in environmental samples. Several references about detecting PAHs in leafy vegetables are listed in the following and I hope it will give you some inspiration. Good luck!
The extraction process depend on what type of compound you want to separate,as you know the second metabolism compounds have different polarities,so you have to use organic solvents with different polarities (like-dissolves-like).Anyway,start with non-polar solvent ( n-hexane or petroleum ether ...etc) then extract with higher polar gradually increase the polarity till methanol-water 70/30.On the other hand the choosing method depends on the required compounds,for volatile compounds (essential oils) should be avoid heating ( use ultrasound bath) for others you can use maceration or soxhlet....etc...Good Luck
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