What is the best medium for culture of cyanobacteria (Mycrocystis or Anabaena) ?

14 May 2022 1 8K Report

I am doing my PhD in cyanobacteria causing off-flavor in the water (Mycrocystis, Anabaena, etc), I found the isolate in the pond (Mycrocystis), but when I cultured and purified the Mycrocystis can't grow, but the other species grow well, I tried in mediums with different N:P ratio, 1:1, 1:5, 1:10, 1:15, 1:20, all show the same result. Anyone can help me, or give me suggestions. Thanks

Imam Tri Wahyudi

Thank you very much Ana T Castro-Castellon , for this good advice.

So far I haven't tried BG11, I have cultured using f2 media with modified NP ratio with aeration, next I will try BG11. Then, for light conditions using a standard lamp but the lux has not been measured, is there any recommendation on how many lux of light is needed, as well as other parameters such as temperature?

Thanks

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Imam Tri Wahyudi's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Method of collection of samples of microorganisms from environmets to labortary?

can any one guid me about tractical method for preservation of microorganism same as bacteria, cyanobacteria and algea, in sampling from environment to Laboratory: 1- Sample collection 2-...

17 July 2024 3,687 11 View

Assistance with high taxonomic level identification of intertidal microalgae in SEM Images?

I am currently conducting research on benthic microalgae in the intertidal zone along the central California coast, specifically focusing on grazing by marine snails. As part of this study, I have...

08 July 2024 3,342 5 View

How can I improve purity of DNA extracted from cyanobacteria using Dynabeads?

I am trying to extract DNA from the cyanobacteria Microcystis aeruginosa using the Dynabeads DNA DIRECT Universal kit for the purpose of on-site detection. Yield is acceptable at ~100-500 ng/uL,...

01 July 2024 10,003 1 View

Which statistical test would be the best?

For my practical project at Uni, I am researching blue-green algae in a freshwater lake. I have three locations around the lake and am testing the water for phosphates, pH, and temperature. I am...

30 June 2024 4,264 3 View

Can biofloc system be applied in earthen ponds?

Dear colleagues Can I apply biofloc system in earthen ponds?

17 May 2024 2,685 0 View

Is anyone willing to do an interview on spirulina cultivation?

Hey there. I'm a Science, Business and Innovation student and for my thesis project I'm currently doing research on different production methods for large-scale cultivation of spirulina....

13 May 2024 368 1 View

Do algae have "vernalization"?

A few days ago, I saw a paper discussing the low temperature "vernalization" of microalgae (cyanobacteria). But in the paper, only low temperatures induced the growth effect of cyanobacteria was...

07 May 2024 2,908 0 View

Why my phb polymer film stick to the beaker after chloroform extraction?

i have extracted cyanobacteria PHB and after some days it was found that the polymer in the beaker became stick and it was difficult to get it from beaker. I want to know the possible reason of...

09 April 2024 5,429 0 View

How to count cells of a filamentous cyanobacteria with a Neubauer chamber ?

I would like to work with Nostoc punctiforme, which is filamentous, and try to assess its growth by cell counting using a Neubauer chamber. Does anyone have experience with this? Is it possible to...

24 March 2024 1,906 0 View