I am looking for M1 macrophage marker that is unique and be expressed only by M1 and not by M0 nor M2. the research papers show different ones like CD80, CD86, CD64, and CD40. The monocytes are from the peripheral blood of healthy donors.
You might try CD115 (c-fms) which is the receptor for M-CSF. I know that mouse M1 macrophage express CD115.
You could have a look at the expression of CD38. It is highly expressed on human monocyte-derived macrophages M1 and not expressed on M0 and M2. Good luck!
You can use a profile of CD markers to characterize M1 cells such as CD 68 a pan-macrophage marker, CD 80 high expression in M1 cells, CD 206 low expression in M1 cells while it is a highly expressed in M2 cells.
you can look up at this article for more benefit.
https://www.nature.com/articles/s41598-017-08121-8
In addiction, the expression of nitric oxide synthase (iNOS) in M1 can also serve as marker.
Mouse M1 macrophages express CD115. But, it is more strongly expressed by a M2/TAM/tissue resident macrophages as use of anti-CD115 mAb treatment depletes TAMs and M2 macrophages. CD38 is a good M1 marker and CD38+ population greatly increases after LPS exposure. iNOS is also very unique for M1 and one of the best. Similarly, SOCS3 is a good marker known for maintaining M1 phenotype and Socs3−/− macrophages had a stable M2 profile suggesting SOCS3 as a regulator of macrophage activation and M1 polarization. TLR2 and TLR4 are other markers to consider.
- Badges
- Science topic
- Similar topics
- Methodology
- Research Methodology
- Research Papers