3 Questions 13 Answers 0 Followers
Questions related from Bianca Maria
Dear all, I´m working with human monocyte-derived macrophages and would like to analyse them by flow cytometry. After monocyte isolation I differentiate them with 100 ng/ml M-CSF for 6 days and...
16 November 2022 8,366 4 View
Dear all, during flow cytometry analysis of primary monocyte-derived macrophages I observe two populations by size (see Image). Is this the usual case when differentiating primary cells in vitro...
12 May 2022 1,721 2 View
Dear all, I´m differentiating human monocytes in vitro using M-CSF and identified a panel of marker that get upregulated in differentiated macrophages including CD68, CD206, CD163, HLA-DR, CD33,...
12 May 2022 3,564 0 View
