I try to measure the enzymatic activity of catalase in a symbiotic Cnidarian,
I homogenized my tissue in 0.1 M phosphate buffer by two methods
1- using fast Prep with beads
2- sonication for at amplitude 20% and even more for longer time period.
then I noticed microscopically that both of theses do not results in significant algal cell lysis
My enzymes are SOD and Catalase,
What is your suggestion. Please
given that the lysis buffer should not affect the enzymatic assays.
Regards
Research Upstream and Downstream for plants cell
you can use both beads designed for animal tissue and plant material, either combine them in a single run, or use them sequentially, following these beads selection guidance: https://www.takarabio.com/learning-centers/nucleic-acid-purification/accessory-selection-guides/sample-homogenization-beads
https://lab.plygenind.com/mastering-bead-selection-for-effective-homogenization
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