In the compressed liquid state, why does the temperature remain constant even if pressure is gradually decreased? But not in the saturated state?

31 May 2020 7,801 1 View

PCR with fluorescent tag on Tecan SPARK?

My boss wants me to run PCR with a fluorescent tag. He says we can use the Tecan SPARK machine that we have for this. I am confused however. Do I run the PCR per usual with the thermocycler then...

15 March 2020 3,765 2 View

Environmental and Occupation Epidemiology Professional Development?

I am nearing the end of my first year of applied epidemiology at a local health department, I have been exposed to a diverse range of topics but throughout graduate school and my project...

05 December 2019 2,430 1 View

NOAA Hourly Precipitation Data Missing Post 2014?

Hello all, I'm trying to test out a predictive model but I'm having a very hard time trying to find hourly precipitation data. I've looked at NOAA and on the new data repository (NCDC) but I...

28 October 2019 4,757 5 View

How can I conduct multiple multilevel meta-analyses using metafor in R?

For my master's thesis, I am using meta-analytic techniques to explore the relationship between LGBTQ community connectedness and indicators of physical health. I am conducting multilevel...

13 October 2019 6,940 3 View

How does one troubleshoot low PCR amplification of soil DNA with ITS primers?

I am looking for suggestions for improving amplification of soil DNA using ITS primers. The soil samples range in concentration from 9-40 ng DNA/ul). Attached are photos of gel confirmation using...

05 February 2019 4,075 9 View

How do I know that I can trust the chemical analysis results of surface and ground water quality samples from a subcontractor?

I was unable to perform the sampling and analysis for 40 surface water and 20 groundwater samples, so I hired an in-country consultant firm to do it. The results that they returned were difficult...

21 October 2018 8,937 10 View

Hello, can anyone recommend which social cognitive model in health can help reduce the risk of hiv contraction ?

Hello, i am conducting a health report for a psychological assignment, this report is based on the reduction of risk in contracting hiv in a prison setting. Unfortunately after reading a fair...

26 December 2017 3,947 3 View

What resources (national organizations, museums, etc) do ESL teachers use most?

If possible please note the developmental stage (early childhood, elementary, middle school, high school) associated with each resource.

03 April 2013 1,073 2 View

Historical importance of the LGBTQ community?

Hi everyone, I'm looking for some articles that discuss the historical importance of the LGBTQ community. I'm specifically looking for theory papers that talk about why a sense of connectedness to...

01 January 1970 7,449 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

02 February 2021 5,008 2 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View

Is there a free software that could calculate electron density on a given atom?

Dear collegues, as stated - I'm looging for a soft that would calculate electron density on a given atom of a small molecules. Either for Ubuntu 18.04 or latest MacOS. I tried multiwfn, but it...

26 January 2021 4,060 3 View