Your query must have been answered in this thread: https://www.researchgate.net/post/How_can_I_evaluate_anti-inflammatory_properties_for_plant_extracts

A very interessting question !

The answer is : According to the method of Winter et al.,animals are subjected to sub plantar injection of 0.1 mL of λ-carrageenan (1% in NaCl 0.9%) into the right hind paw . Then, they are subjected to a gavage with 0.9% NaCl at 10 mL/kg; or sodium diclofenac at 50 mg/kg as reference drug, and extract of Plant or bioactive compounds. IV: the same extract at 250 mg/kg. and Diclofenac, both dissolved in 0.9% NaCl, were administered by oral dose 1h following carrageenan injection. The MEPaL was first evaporated under vacuum at room temperature to remove the methanol, and then the deposit was solubilized in a 0.9% NaCl solution.

The circumference of paw will be measured at h1.5, 3 and 6 after carrageenan injection. Increases in the linear circumference of the right hind paw were taken as an indicator of paw edema. Percentage increase in edema (%IO) will be estimated in terms of the difference in the zero time (C0) linear circumference of the injected right hind paw, and its linear circumference at time T.