Please, specify, column, flow rate, mobile phase and wavelength.
Using UV-Vis as a detector.
A standard C18 column should retain benzalkonium chloride and resolve the different chain lengths. I got better results and less carryover using methanol as mobile phase B. Carryover can be a real issue with this compound.
Seriously, why are the buttons on the left? (Photo: Timur Emek/Getty Images)If you’ve ever compared a woman’s button-up shirt to a man’s, you’ve probably noticed that the buttons are on the...
05 June 2016 2,676 9 View
During the past three months I have noticed that some research gate members asked questions, however, they never commented on their peers answers, & others asking questions that they know...
01 February 2016 5,516 20 View
We have designed and synthesized a number of novel amoxicillin and cephalexin prodrugs and looking for somebody to carry out the in vivo studies for determining their pharmacokinetics parameters.
10 November 2015 8,403 6 View
We have designed and synthesized a number of prodrugs for commonly used drugs and looking for a place to conduct toxicity tests for these novel compounds.
10 November 2015 1,191 4 View
Please, write all the procedure steps.
10 November 2015 9,494 11 View
What basis set to use?
10 November 2015 1,649 0 View
What basis set to use if the size of the organic cation is 50-65 atoms including hydrogens?
10 November 2015 3,349 3 View
Please, specify column, flow rate, mobile phase and wavelength.
10 November 2015 6,986 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
I imagine the battery drives the graphite into a carbocation, then chloride is oxidized to donate the electron to the carbocation, but what pushes the lost electrons of chloride to be shared...
03 August 2024 5,384 0 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View