I am looking to inhibit calpains using calpeptin treatment. I have seen many concentrations of calpeptin used in different manuscripts but wanted to see if anyone had experience (good or bad) with certain concentrations. Thanks.
So were we wrong about acid rain in the 1980s? Can we be sure that the new story: Climate Change, Desiccated Soil, and Monocultures, is correct? Another alternative from the 1980s is the massive...
10 July 2024 4,667 7 View
I would like to perform a literature review at this time on augmented learning and learning augmented algorithms to enhance performance-guided surgery
06 July 2024 246 1 View
I have a number of peer reviewed articles that were published that might be useful on this site. I don't know how articles appear on this site, but I would upload mine if acceptable. Thanks.
23 June 2024 2,344 1 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
05 June 2024 624 2 View
My research group has samples of plant tissue that were pulverized in ln2 using a mortar and pestle before being weighed into tubes and stored in RNAlater at -20 degrees for a few months before...
28 May 2024 7,274 0 View
My research, in particular my analysis of time perception, suggests that consciousness correlates with a large scale quantum coherent state in the brain in the form of a Bose-Einstein condensate....
16 April 2024 2,819 0 View
I ask this question from the perspective that AI algorithms can automate tasks, analyze vast amounts of data, and suggest new research avenues. It can also improve research efficiency and speed up...
15 April 2024 9,158 8 View
Introducing Artificial Intelligence in the first year of engineering studies offers students a foundational understanding of its principles and applications. This early exposure fosters relevance,...
11 April 2024 3,934 2 View
Humans have the longest childhood of any animal known. There are two huge growth spurts in our lives where cascading hormones shape our bodies. Infancy and adolescence, with the same surges of...
08 April 2024 2,355 22 View
ANY SCHOLAR WITH A SIMILAR WORK OR HAVE INTEREST IN THE ABOVE TOPIC?
23 February 2024 4,328 3 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
13 July 2024 2,255 3 View
There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.
08 July 2024 4,988 1 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
04 July 2024 8,100 4 View
For the measurement of electrode reaction kinetics and electron transfer rate constant, using E. Laviron's model and RICHARD S. Nicholson's model, the greater peak potential separation indicates...
30 June 2024 580 3 View
I am performing catalytic metal supported benzyl alcohol reaction kinetics in presence of potassium carbonate base. Every time order change observed at lower catalyst conc. It showing zero order...
20 June 2024 7,100 2 View