Hi Silvana!

In the lab we use the OTA (BACHEM: d(CH₂)₅¹,Tyr(Me)²,Thr⁴,Orn⁸,des-Gly-NH₂⁹)-Vasotocin trifluoroacetate salt ) in a concentration of 1uM for brain slices (amygdala, prefrontal cortex).

Best!

Rodrigo

Thanks a lot for you answer, Rodrigo! Have you tried other inhibitors? We also use OTA at 1uM in our lab (although I haven't personally used it yet) but haven't tried anything else. I've seen both OTA and L-368,899 being used for in vivo infusions but didn't find anything about L-368,899 in slices.

Hi Silvana,

We have not used L-368,899 , neither in vivo nor in slices, we do have used atosiban (Deamino-Cys1,D-Tyr(Et)2,Thr4,Orn8)-Oxytocin ), in slices, but it is not a good antagonist, as it can work as positive allosteric modulator of the OTR. Then, we do not recomen it as antagonist.

Best,

Rod