Transformation of Bacillus subtilis?

Colonies appeared after transformation of pHT01 plasmid into Bacillus subtilis, and band was confirmed by performing colony PCR. However, if the colony is picked and grown (in LB+antibiotics) and...

27 October 2020 609 4 View

I keep getting an error for GEOPIV on Matlab?

When doing the geoPIV_RG_run, i keep encountering this error. Error using dataread Trouble reading floating point number from file (row 1, field 2) ==> Dynamic Load Test Free Vibration Error...

20 October 2020 10,004 2 View

Is receiving a review invitation of a paper that has already in the second revision normal?

I am new to the "reviewer world". I received an invitation to review a manuscript for one of the open access journal from IEEE. When I download and open the manuscript, I realized that seems...

20 May 2020 316 13 View

How do I dissolve methanolic plant extract completely without affecting results?

I work on plant extract for seed germination assay. However, I face some issues on dissolving my plant extract. Initially, I try to use distilled water to dissolve it but my plant extract is not...

18 December 2018 4,479 2 View

What are the commercially available materials that has a high surface area or CEC (cation exchange capacity)?

The material (besides clay) needs to be non-reactive/stable, and preferably synthetic. Thank you very much in advance! Your suggestion is very much appreciated!

08 December 2018 2,265 6 View

If the aspect ratio (length/diameter) of natural fiber is too small, and the results obtained is just an increment of 5%, what is the length possible?

if the aspect ratio (length/diameter) of natural fiber is too small, and the results obtained is just an increment of 5%, what is the length possible? This is according to the aspect ratio of 40%,...

22 February 2018 9,281 8 View

What we can learn from a well tuned PID gains for unknown plant?

I have a closed loop biological control system where i use a PID controller. Because the transfer function of the biological system is complex to derive, I used trial and error to tune the PID...

05 October 2017 4,434 2 View

What are the difference(s) in Inconel 718 conforming to two different specifications? One is for aerospace and another for oil and gas industries.

AMS 5596K and AMS 5663. What is the equivalent of ISO 15156 for aerospace application?

04 July 2017 5,541 3 View

Is there any preference (load controlled or strain controlled) for high temperature fatigue testing, say 600 degree celcius?

Nickel based superalloys

03 July 2017 2,897 12 View

Mouse hepatocyte isolation with percoll?

I had practiced mouse hepatocyte isolation with percoll for several month. The primary hepatocyte cells can only survive one day or less, and the cause of cell death is DNA released....

15 January 2017 216 0 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View