I am going to evaluate the efficacy of growth promoting substances through foliar application against salinity. if i have to spray only once at what growing stage of wheat i should do it?
It is of, course, germination, but one cannot deal with any adverse effects of salinity by foliar application in this particular stage. If cultivated plants are models, then it would be logical to spray either relatively early in vegetative stage when plant is actively growing or preceeding the stage of most active yield formation. This phases should be specific for a particular crop.
I think it will much defect in a log phase of vegetative growth. You can predict the period using sigmoid models. The rate of plant growth is represented by sigmoid models.
Which phase of plant life cycle is more sensitive to salinity is different among species and cultivars and specific for a particular crop. In order to alleviate the salinity effects through exogenous application of hormones and others, I would suggest to try seed priming with different times and concentrations. In addition, foliar spray at seedling stage would be helpful. I think it is better to have a trail experiment in which different protocols can be tried and then decide.
Also, you must consider, reaction time of plant to different stresses is important. for example seedling of olive needs 45 days to act to salinity stress.
it also depend on different plants types like in rice crop heat stress influences more reproductive stage than vegetative. similarly for salinity some time it adversely affect germination and some time vegetative and reproductive stages