Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

Anyone has an experience extracting proteins from rectal swab samples?

I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...

02 February 2021 3,622 3 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

Cross country comparison with matched pairs design?

Hi, I'm doing a cross-country comparison. My data for each country has a match pair design i.e. each company within a country is match with a control firm from that country. The data is...

07 January 2021 4,983 6 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

How to generate topology file for ligand for Gromacs?

Dear all, I converted a PDB file into Mol2 file using Openbabel (H added) and then fixed the bond order number using this command: perl sort_mol2_bonds.pl jz4.mol2 jz4_fix.mol2 When I try to...

22 December 2020 303 4 View

Can we used PTOTACs to improves the RNA vaccines immunity period ?

Using targeted degradation of non structural proteins of viral capsid can we lengthen the vaccines effectiveness

21 December 2020 7,200 1 View

Stably transfected A549 cells are getting washed off during a plate based assay?

HI, I have transfected A549 cells with a plasmid of interest. The transfectants have been stably selected and clonally separated. I am doing a plate based fluorescence assay with the transfectants...

20 December 2020 1,222 4 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Which software is the best to model a non-linear concrete (ABAQUS, ANSYS,ADINA, ATENA, etc.)?

What is the best finite element modeling software packages for modeling non-linear and fracture analysis of CONCRETE? I've had some with ABAQUS standart/explicit, but I would like to learn if...

27 January 2021 330 6 View

Do we need to keep looking for the fracture angle after the Puck's failure initiation is reached?

Greetings to everyone, Our team is currently working on the development of a VUMAT code in Abaqus for composite modelling using the Puck's failure theory as damage initiation criteria. Currently...

20 January 2021 6,367 1 View

Fracture energy of concrete at sub-zero temperatures?

Both tensile and compressive strengths of concrete increase with the decrease in temperature. With this in mind and considering that fracture energy is in direct correlation with the so-called...

18 January 2021 8,778 4 View

Permeability of fractured reservoirs?

Hi all, What I know the permeability of matrix and fracture can be in a broad range in fractured reservoirs. Am looking for the most usual range for their permeability separately. I will be highly...

15 January 2021 5,271 2 View

Why the fracture energy of concrete increases with the rise in temperature?

Given the degradation of mechanical properties in concrete under elevated temperature namely, tensile strength, modulus of elasticity, compressive strength, etc., what is the justification behind...

15 January 2021 3,651 4 View

How does a cell repair itself? Can you relate this to mathematical genetic algorithm?

I am on a quest to solve how a cell repairs itself through encoding-decoding of proteins. Is there any link to genetic algorithms to solve age old questions such as aging and how we heal?

14 January 2021 9,958 1 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View