biology
I want to extract an extracellular protein from tissue to be further subjected to gel filtration and subsequently mass spec in other to identify potential interaction partner. As this protein is...
08 September 2018 353 3 View
Does anyone have a working protocol on immunoprecipitation of tissue extracts? This is aimed at further concentrating the protein of interest for easy detection on western blot.
01 January 1970 7,657 10 View
This is required in other to reverse transcribe the mRNA to generate the required cDNA for PCR in the process of cloning
01 January 1970 2,126 2 View
What might cause a recombinant protein not to be secreted to the extracellular matrix otherwise getting stucked in the cell. This is a domain of a recombinatly expressed extracellular protein and...
01 January 1970 5,055 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...
02 March 2021 4,119 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
