Temitope Esho

5 Questions 9 Answers 0 Followers

Questions related from Temitope Esho

How can one better extract an extracellular Matrix Protein in large quantity from a tissue of interest?

I want to extract an extracellular protein from tissue to be further subjected to gel filtration and subsequently mass spec in other to identify potential interaction partner. As this protein is...

09 September 2018 411 3 View

What is characterization in molecular biology?

biology

08 August 2016 262 0 View

Does anyone have a working protocol on immunoprecipitation of tissue extracts?

Does anyone have a working protocol on immunoprecipitation of tissue extracts? This is aimed at further concentrating the protein of interest for easy detection on western blot.

01 January 1970 7,702 10 View

Which organ/organs are frem family proteins expressed in experimental mouse?

This is required in other to reverse transcribe the mRNA to generate the required cDNA for PCR in the process of cloning

01 January 1970 2,162 2 View

What might cause a recombinant protein not to be secreted to the extracellular matrix otherwise getting stucked in the cell ?

What might cause a recombinant protein not to be secreted to the extracellular matrix otherwise getting stucked in the cell. This is a domain of a recombinatly expressed extracellular protein and...

01 January 1970 5,084 3 View