I am confusing about the 'time of formation' of nanoparticles in green synthesis method after addition of plant extract with precursor solution. From the previous reported literature, we can identify the presence of expected nanoparticles by changing the color and by strong absorption peak in UV- Vis spectrum. I want to know that when we get the expected color of nanoparticles and still we continue stirring the solution for more time, then what will be the particular definition about the time of formation of nanoparticles?

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