What is best vector and Expression host used for lytic polysaccharide monooxygenases ?
I need a pdb file of the following ligand : tetrasaccharide-pentapeptide NAM-NAG-NAM(-L-Ala-D-iGln-L-Lys-D-Ala-D-Ala)-NAG . It is part of the peptidoglycan polymer
03 April 2019 7,134 4 View
Enzymes can have stabilization effect on soil particles. The effect is due to reduction of the electrical double layer around the soil particles as a result of cation exchange effect. Another...
05 June 2015 7,462 1 View
There is a m.o. that noticed conversion of a broth into gel. The medium is starch + carbonate .
03 April 2015 5,202 2 View
I have a sequence of a (putative) glycoside hydrolase enzyme , I want to do a phylogenetic analysis to get information about its class and anticipated activity. Which online server can do this...
31 December 2014 3,779 2 View
What are trustable commercial laboratories doing protein identification and purification.
11 December 2014 629 4 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
Hi, I have grown BL21 cells with the pET28a that carries my insert sequence. It is growing on the selection plate but I can't observe any insert sequence bands when I do colony PCR. Is there any...
15 July 2024 5,135 5 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View