drying down a prep hplc fraction to powder. How to debuffer the fraction and convert it in to powder for characterization and preclinical studies.
Dialysis and a rotary evaporator. After purification, you may utilize freeze drying as final step, if desired.
I have 99% DMSO bottle. Density 1.1g/l and MW 78.13 g/mol =0.99*1100=1089g/l=1089/78.13=13.9M After calculation stock solution will be 13.9 M. 71 ml in 1000ml gives me 1 M 1 mol/l*78.13g/mol =...
02 March 2016 6,210 2 View
I am trying to calculate my dose for a substrate for a particular enzyme whose Km value is 0.8mM. can i directly calculate based on molarity or any other way I am new to this so looking for some...
31 December 2015 7,137 6 View
I am trying to understand Emax model for calculation of EC50 from plasma drug concentration against time. I am trying to google it but unable to find what i want. Every where they have used conc...
05 June 2015 3,560 2 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View