Hello,
I recently isolated protein from adipose-derived stromal vascular fraction. I hope to use this for immunoblotting. Is there a marker to sort of confirm that this fraction isn't contaminated with MATURED adipocytes?
You could sort out the matured adipocytes instead? That would leave behind the cells you are interested in. AP2, CD36 and PPAR-gamma could be used...
I've been trying to design some qPCR primers lately. I am aware that the ideal primer should span an exon-exon junction. However, two of my gene of interest (GPR109a and GPR40) have only one exon....
04 May 2016 7,702 8 View
a second update to the checklust of the vaSCULAR FLORA NATIVE TO ITALY
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I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
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Dear researchers. I have extracted data from graphs for meta analysis. I am stuck to find the SD for the obtained data. Anyone who can help me to sort out this problem with formula? Thank...
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Greetings all, My group has been trying to reproduce 3t3-L1 cell (preadipocytes) differentiation. The typical protocol involves 3 steps: the first step and second step both last for 2 days, and...
18 June 2024 2,728 1 View
Endoform™ Antimicrobial is indicated for the management of acute and chronic wounds including partial- and full-thickness wounds, pressure ulcers, venous ulcers, diabetic ulcers, chronic vascular...
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I have a longitudinal dataset where I am looking to examine whether individuals change their category membership of a variable (with four categories) over four time points (baseline, 1y, 2y, 3y)....
16 June 2024 9,770 10 View
Porn to men is not a big deal. They honestly can’t see how watching a sexy film can be any sort of reflection on their love for their girlfriend. Most don’t understand why she takes offence...
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There are two proteins X and Y both containing signal peptides. We want to create a fusion protein of X-Y. X will be displayed on the surface so we retained the signal peptide of X. Further, do we...
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Why it is not possible to sort my research items according to number of their citations?
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While performing gromacs MD simulation after pdbtogmx command using charmm force-field, i have received "Atom P1 in residue NDP 193 was not found in rtp entry NDP with 75 atoms while sorting...
02 June 2024 9,660 8 View