I want to quantify my drug using HPLC, but the the solvent im using (DCM) is interfering with my readings at 360nm. What is an alternative solvent to use that is not excited at 360 nm? Thanks.
Hello Muhanad,
I suppose you may use ethanol which does not emit at 360nm.
Have a look at some of the common solvents that you can use in the attached graph. Hope that gives you an idea.
You need to use a solvent or mobile phase that the sample(s) is soluble in AND which does not absorb in the same range as your detection wavelength. Ethanol may or may not be the one, and your decision which one to use should be based on the previous statement.
Are you using Fluorescence detection (FLD) and/or UV/VIS? You used the word "excited" which makes me think that either you are very excited about your project or you have another issue with choosing a good excitation wavelength for your detector. More information (details) about the exact sample and method would help us provide you with more constructive advice.
Terminology is key: You used the phrase, "high emission", but I think you really meant to say UV/VIS absorption. Mobile phase choice effects this directly.
In the meantime, here is a link to a very comprehensive table of "HPLC Solvent Properties" which includes UV/VIS cutoff values [ http://www.hplctools.com/lcsolvent.htm ].
http://www.hplctools.com/lcsolvent.htm
Thank you for your responses Vidhura and Bill. I'm using UV/VIS to detect my compound at 360 nm.
I think 2-methyl tetrahydrofuran could be a good replacement for DCM in HPLC.
you can find more information by follow this link.
http://www.pennakem.com/pdfs/methfdichloromethane3.pdf
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