How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How to increase bacterial concentration of DNA from Laser capture microdissection samples?

I used laser capture microdissection, selected specific bacteria presented in ethanol-fixed slides. And DNA was extracted by Qiamp mini kit. The problem right now is we have pretty low...

09 February 2021 3,908 2 View

Problems in Simulation of Carbon Dioxide discharge from pipe using fluent (Read description below)?

I am trying to simulate carbon dioxide discharging from the pipe using ansys fluent and want pressure variation with time inside the pipe. The geometry consists 144m long pipe and 146.36mm inner...

25 December 2020 1,533 3 View

How can I determine the mass of a refrigerant (for example R744) in a evaporator when the refrigeration system is not working?

By knowing the specific volume on the inlet and on the outlet, is it correct to assume an average of those two specific volumes?

30 November 2020 3,807 3 View

Is it possible to add hierarchical porous Cu/Zn-nanoparticle@carbon materials into a carbon paper based gas diffusion electrode (GDE) ?

26 November 2020 5,075 1 View

Does anyone have experience with the Nicoya OpenSPR COOH sensors?

Hi, I am planning on running some protein-protein interaction/kinetics studies using the Nicoya COOH sensors in OpenSPR. I am looking for the activation and blocking buffer composition that are...

01 November 2020 262 1 View

Should I include irrelevant parts of my methodology?

I am writing up a project in which I used motion capture as well as heart rate monitoring and questionnaires, but I will be analyzing the motion capture data separately to this project write up -...

23 October 2020 6,796 5 View

Is there a database indicating the carbon and nitrogen storage capacity of vascular plants (in Europe)?

Hi everyone, I search a database or a review listing the capacity of vascular plants to storage carbon, nitrogen or other important chemical elements. I work actually on a mountain area (Swiss...

19 October 2020 8,596 3 View