I have not performed this assay.

But the attached review paper discusses the various methods for NO assay.

Maybe this maybe of some help to you. Best of luck.

I have tried Griess reaction and it works with me but there are few things to be taken into consideration 1- this enzyme is unstable it loose( when it is in crude system i.e. in plasma ) most of its activity within one week.2- more than one form of the enzyme are present in the body and the induced form need the presence of calcium ion ,so you can not detect its activity if you use any chelating agent like EDTA..

This protocol will be useful..

Hello, thanks for the protocol.  Has anyone tried this protocol, but with reduced volumes??  I'd like to do everything in a flat-welled 96 well plate, and use much less of my compounds.  I will do the following: 100µL of nitroprusside with varying concentrations of scavengers, then add 100µL of Greiss reagant.  Also, can someone post some precise product information for a greiss reagant that works well for this assay?

You can please refer to the following links to get certain valuable details:-

1. http://www.thepharmajournal.com/vol1Issue12/Issue_feb_2013/9.1.pdf

2. ijpba.info/ijpba/index.php/ijpba/article/download/412/291

3. http://jocpr.com/vol3-iss4-2011/JCPR-2011-3-4-647-654.pdf

4. http://www.iosrphr.org/papers/v3i2/Part_2/H0322038047.pdf