How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Energy minimization of a protein structure with acetylated lysine residues?

I performed in-silico acetylation using PyTM plugin but there are certain clashes that I want to resolve. I couldn't perform energy minimization using gromacs, chimera as they do not have default...

26 September 2020 3,907 6 View

Any ideas why i might be experiencing loss in fiber weight after acetylation?

In recent experiments acetylating cellulose fibers extracted from the leaves of a particular plant, weight loss was experienced. Experiments were repeated several times and reaction mixture was...

18 March 2020 4,599 3 View

Why am I unable to detect Histone PTMs at their correct molecular weight in nuclear extracts via Western Blotting?

I am trying to detect changes in Histone PTMs with or without treatment in nuclear extracts from cell lysates. I use a Hypotonic buffer (20 mM Tris pH 7.4, 10mM NaCl, 1mM MgCl2, 0.5mM EDTA)...

10 March 2020 2,626 6 View

How to perform a MD simulation of a protein with post-translational modifications in Gromacs?

Hi all, I am planing to perform a MD simulation of a protein with two acetylated lysines. Which workflow do you recommend me to follow? Has anyone used Vienna-PTM 2.0? any preferred force...

06 July 2019 8,471 2 View

What are the ways of determine a cognitive changes in muscarinic acetyl choline receptors activation?

Am doing a comparative study of some plant extracts. Pls What are the ways of determine a cognitive changes in muscarinic acetyl choline receptors activation?

05 June 2019 7,098 2 View

Western blot of acetylated EP300/CBP proteins?

Hey all, I am new to WB and I am trying to detect the acetylated form of EP300/CBP but I get extremely faint bands and also almost a smear of unspecific bands. I have tried leaving the Ab...

04 May 2019 7,878 4 View

What could be possible undesired reactions if I start Fmoc removal by PIP/DMF if I had an incomplete washing of the acetylated (AC2O) peptide?

I happened to start Fmoc removal of Peptide using 18,6% Piperidine in DMF before next coupling when the peptide was not well washed after acetylation by acetic anhydride. Could there be any...

02 March 2019 8,038 4 View

Is it possible that acetylation of lignin can result in its solubility in ethanol?

I acetylated lignin and lost all of its precipitate while washing with ethanol

29 January 2019 4,296 3 View

What is the difference between ESI-MS/MS and LC-MS/MS?

I will like to detect and quantify acetylation in Histones and RNA. Which one is preferred?

31 December 2018 8,152 7 View

Could anyone suggest condition for PPD to react with aldehyde, since acetylation of PPD is giving poor results?

Since PPD(p-Phenylenediamine) is a diamine, While doing acetylation of PPD, we are getting multiple byproducts.

31 December 2018 5,869 12 View