How to relate relaxation time values obtained from different equation?

I tried to fit the frequency vs Dielectric constant and f vs Dielectric Loss graph with HN equation. I got different Tau (relaxation time) values from the fittings of both graphs. What I have to...

12 January 2021 8,467 6 View

Can anybody tell me about the basic principle behind the use of neutral and alkaline comet assay ?

In most of the research paper people used both neutral and alkaline comet assay to determine the level of DNA damage either single stranded or double stranded but the exact principle of both the...

02 August 2020 2,193 2 View

Is it possible to provide online education to all the children studying in the schools during this lockdown period?

In many countries, the children belong to remote villages and do not have facility of computer or Android mobile and internet connectivity. Do you think, the countries will be able provide online...

17 May 2020 2,739 16 View

DFT calculation of Diradical Singlet in Turbomole?

Using TURBOMOLE, I am studying a mechanism of Heterolytic vs Homolytic cleavage. When the bond breaks, there are three possibilities a) Heterolytic Cleavage (RHF, singlet) b) Homolytic Cleavage...

15 October 2019 4,214 5 View

How to obtain the MODIS vegetation index for a particular date which lies within the 16 day composite ?

I want to obtain the vegetation index map for a date which lies in between the 16 day composite MODIS vegetation indices. Ex- Let's say the MODIS (both terra and aqua) has 16 day composite images...

01 September 2018 9,489 6 View

Which predictive model would be the best one to design a scoring model for Big data?

Logistic regression, SVM, andom forest etc

24 July 2018 1,019 4 View

What could be a suitable jet polishing solution and parameters that I can use for TEM studies of precipitates in 718Plus?

I have come across a lot of journals with different solutions. But I am not sure which one can be used. Please suggest.

19 July 2018 1,903 1 View

How increase in flexural strength can be compared with decrease in flexural modulus ?

In detail, I performed flexural test of some composite specimen. I got higher flexural strength with high modulus. But after doing some modification in main material, I got enhanced flexural...

15 April 2018 4,826 7 View

Could anyone suggest some articles/ reviews which show MHC II expression of murine monocytes and macrophages in different tissues during inflammation?

I am looking into monocytes and macrophage in murine model (C57Bl/6) of a disease.

22 March 2018 959 2 View

How graphene can be coated uniformly on the substrates?

Which technique is preferred in this case? Spin coating technique or spray technique.

18 March 2018 1,625 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View