I am looking for recommendations about a sensitive but a simple and non-expensive method (a kit) for the evaluation of the magnitude of oxidative stress in cyanobacteria/bacteria.
Thanks, Igor .
Part of interisting information for you might be found in the new research: Components of Antioxidant Systems in the Cells of Aerotolerant Sulfate-Reducing Bacteria of the Genus Desulfovibrio (Strains A2 and TomC), Isolated from Metal Mining Waste. 2016, A.L. Bryukhanov et al. If you need furher details, please, connect with me.
Dear Dr. Brown,
You can go through the attached paper for some ideas.
As you would see Oxidative stress is a global phenomena, where excess amount of various reactive oxygen species (ROS) will be produced. In cyanobacteria, being photosynthetic, these ROS will be produced from both photosynthetic and respiratory electron systems. As a defence from these ROS, cyanobacteria will respond both through antioxidative enxymes (eg. superoxide dismutase (SOD), catalase (CAT) and/or peroxidase (POD), etc.) and antioxidative compounds (glutathione reduced, ascorbate, ergothioneine, carotenoids, etc). SOD is considered as first line of defence that works in association with CAT/POD to neutralise the stress, hence I would assay these enzymes first (either by Native PAGE or spectrophotometrically), then obviously I would characterise carotenoids content and types (if possible) to understand the oxidative status of the cells. Other enzymes such as ascorbate peroxidase, glutathione peroxidase and glutathione S-transferase activites are also important to look for. Likewise, ascorbate, glutathione and ergothioneine levels also may be very good indicator apart from carotenoids content.
Thanks
Sushanta
DearDrs Bhattacharyya and Saha: Thank you very much for your information and suggestions. IB
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