Hello everyone,
I am interested in ordering a modified DNA oligonucleotide such that it's terminal 3'-OH is blocked in a way that it can be unblocked. Or a dNTP with such a modification that can be added to the 3' end of an oligo. The problem is that this sort of modification is either very rare, or I am uninformed and having trouble finding it. Does anyone have any suggestions for such modifications that are commercially available?
An example would be allyl esters or alkoxylamine groups, or anything that is used in NGS sequencing. In illumina for instance there is a 3'-O blocking group that stops synthesis, the color of the dye is read, and the the blocking modification is removed chemically so that the process can repeat.
Thanks in advance for the answers.
Best regards,
Deni
Do you need the removable 3'-blocked dNTPs also labeled with dye or no label?
If the removable 3'-blocked dNTPs alsolabeled with dye , they are the detactable sequencing terminators used by Illumina/BGI.
Guobing Xiang Do you happen to know any company that provides this kind of oligos?
Gary
I am not sure the oligos with 3'-blocked are commercially available, but I think they can be made by post-synthesis, add 3'-blocked dNTPs on the 3'-end of the oligos. with enzyme. Or they also can be made by regular DNA synthesizer with special phosphoramidites(with universal support) or with 3'-blocked dNs on the support.
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue