Internal standard can be used for the analysis of amino acids and organic acids by HPLC is caffeine.
Instead of an internal standard you could determine that response factors for each of the compounds that you are analyzing. Everything will depend on the nature of the samples you're studying.
Internal standard should always be similar to the analyte but be very much distinguished. e.g. for determination of organic acid like octanoic acid, decanoic acid, dodecanoic acid, tetradecanoic acid, etc. one can use tridecanoic acid, pentadecanoic acid as internal standard. This is because 1. tridecanoic acid is of same nature to that of other acid. 2. It can be clearly distinguished from other even similar type of acids 3. In nature odd carbon acid are rare.
Similar is the case with amino acid or any other analyte even for HPLC/GC or any other separation technique
For LC-MS analysis, labelled amino acids and organic acids are best. However, for other types of detectors, analogous compounds (belonging to the same chemical
class but not present in your samples) can be used.
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