I have searched literature and am aware most HEK cells have little to no expression of TLR4, so no LPS stimulation. We tried using TNFa and CD40L, but no luck. I was wondering if anyone has experience with this specific type of cell and was able to get a measurable induction. Or perhaps a source where I can look up cell surface receptors of this specific type of cell?
would greatly appreciate the help :)
Hello Lobna Elsayed
According to the transcriptomic data reported in the study in the paper attached below, no gene expression data for the main LPS receptor TLR4 in the FreeStyle 293 cell line was detected. However, transcriptomic data for other proteins involved in LPS signaling for example, LBP, MyD88, TIRAP, CD14, IRAK1, TRAF6 or TICAM 1 were clearly detected, albeit at low level.
In contrast to TLR4, TLR1 gene expression was detected in all analyzed HEK-293 cell lines. TLR1 is activated by binding to diacylated and triacetylated lipopeptides. This article presents genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines.
https://www.nature.com/articles/s41598-020-76137-8
You may also want to refer to the article attached below.
Article Impact of lipopolysaccharides on cultivation and recombinant...
Hope this helps!
Best.
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