I want to impose drought by withholding water for about 20 days on 20 day old plants. I am working on oat and need to look at root traits.
Dear Krishna Ghimire ,
You can supplement MS or 0.5MS with polyethylene glycol with a molecular weight above 6000 (PEG-6000 or PEG-8000). Its molecules are inert, non-ionic, non-plasmolyzing and cell impermeable.
It is widely used to simulate drought stress. Yet, I would think about the difference between osmotic and drought stress, as in the first water is present, but not available.
Article Clarity on frequently asked questions about drought measurem...
To assess pattern of root growth, sand is the best growing medium. Yet it is not a preferred growth substrate for most plants such as the one which i am working on i.e. rice. The soil volumetric water content of drought pot should also be meaured to ensure the attainment of final target drought intensity (measuring time: 8:00–10:00).
For growing medium in drought experient send is use for root system
To assess the pattern of root growth, sand is the best growing medium. You can use PVC pipe 1 to 1.5 feet in length (3-4 inches dia), fill them with sand and place them in plastic bottles.
Hi
I used soil with medium sizes such as sandy clay loam, sandy loam, or silty loam in the lysimeter experiment because those textures are an indicator of suitable soil for the good establishment of the plant, especially woody plants. Roots in this soil can better growth and development, air and water better transudation.
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