How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

How do you integrate the 'friction' variable Xi in the Nose - Hoover Thermostat in order to apply it to a molecular dynamics simulation?

Everything tells you the derivative of Xi, but not how integrate the value to implement it into a simulation. How do you do this?

21 February 2021 6,481 1 View

How to implement boundary conditions in Abaqus contact problems?

Hello I am trying to simulate crawling motion of a bilayer beam using Abaqus. Kind of similar to this: https://youtu.be/os-Z3ucoFpA I am using multiphysics enabled (diffusion, deformation,...

20 February 2021 2,916 1 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View

Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 605 4 View

Can anyone help me with the problem of hyperelastic coefficients in abaqus?

Hello Everyone, I have done a uniaxial test on rubber and data (attached with the post) has been used in Abaqus to get YEOH's coefficient. But I am getting a warning that it is unstable? Can...

04 February 2021 5,975 3 View

When to use Darcy or Fanning friction factor in frictional pressure drop measurement?

04 February 2021 6,174 3 View

Inquiry regarding engineering journals for free publications for my dissertation (laser polishing of 3D printed parts?

my dissertation was on laser polishing of 3D printed plastic parts, i want to publish it, can anyone suggest me a free copy engineer journal for this purpos, plz?

01 February 2021 7,140 4 View

How to measure the hardness for PLA & PHAs polymers?

Hello, I am a bit confused on whether to follow ASTM D785 (Standard Test Method for Rockwell Hardness of Plastics and Electrical Insulating Materials) or ASTM D2240 (Standard Test Method for...

21 January 2021 7,487 4 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View