I am creating nanoliposomes to use as a delivery method to mix hydrophobic pigment zeaxanthin with hydrophilic protein GSTP1. My faculty adviser wants to use FRET to help measure how well GSTP1 moves zeaxanthin from donor liposomes to acceptor liposomes. However, we do not know what fluorophore will work with this assay, and I do not know how to find an appropriate fluorophore.
Thanks for your time.
Can you supply fluorescence emission spectra for zeaxanthin in liposomes? This is necessary to decide on a suitable acceptor.
Hi,
Have a look at the following papers, you may find them useful:
Fluorescence Resonance Energy Transfer in Polydiacetylene ... - NCBI
https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
by X Li - 2008
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The design and application of fluorophore–gold nanoparticle ... - NCBI
https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
by M Swierczewska - 2011
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monomeric fluorescent protein: Topics by Science.gov
https://www.science.gov/topicpages/m/monomeric+fluorescent+protein.html
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Applied systems biology - DTU Orbit
orbit.dtu.dk/files/53263898/j.1742_4658.2010.08705.x.pdf
by E Moravcikova - 2012
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