What factors are to be considered in designing mutants to knock out the salt bridges of a humanized antibody fragment(fab)?
I do not know what salts you are using. I would recommend cold ethanol, or cold isopropanol type of solvents to be used in place of the salts you are using.
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
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