What dye is safe to add to the ACSF inside glass electrodes when recording in-vitro LFPs?

01 January 2019 1 6K Report

Hi all,

I've been learning to record LTP in in-vitro mouse hippocampal slices on an LFP rig. I have trouble seeing the very fine glass electrode tip (I pull 2 megaohm electrodes on the pipette puller) so my PI gave me some Chicago Sky Blue to use to color my ACSF. It works really well, but I just found out that it is a potent VGLUT inhibitor. Will the small amount that may leak from the electrode tips affect my LTPs? Does anyone else use this dye, or another one, to help increase visualization of your electrode tips? Thanks in advance!

Jacci Newel

Thanks so much, Michel K Herde ! I don't need a fluorescent dye as I'm just using a stereomicroscope with a backlighting set-up, equivalent to brightfield illumination. Apparently I am just blind compared to my colleagues, as I don't think it is standard practice to dye your recording electrode ACSF for simple LFP experiments! ;-)

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