-The statics analysis is wrong, the experimental design as well as the scientific question cannot been answered by a one-way ANOVA. Also, a couple of control groups are missing.
Thanks a lot for your help. Kindly my statistical Analysis:
All obtained data were reported as mean ± S.D. The results were examined using one-way ANOVA, followed by Tukey's multiple comparison tests. When normality failed, non-parametric one-way ANOVAs (Kruskal–Wallis one-way ANOVA on ranks, followed by Dunn's post hoc test) were done and when homogeneity of variance failed, Welch’s ANOVA and differences between two groups were analyzed using the Games-Howell test. Statistical analysis was carried out using SPSS software (version 26) (IBM Corp., Armonk, New York). The threshold of significance was set at p 0.05 for all statistical tests, and graph pad prism software was used to present the graphical design (version 8) (San Diego, CA, USA). F-value (F), degrees of freedom (df) and statistical significance (p) were reported.
Since the statistical analysis was conducted using appropriate methods and software, and the results were reported with relevant statistical parameters and significance levels, there does not seem to be a major concern with the statistical analysis itself. It would be helpful for you to address the concern raised by the reviewers in their response, and to provide a rationale for your choice of using standard deviation in the graphical representation of the results. Based on the nature of the data and the analysis, you could also consider providing additional analyses using standard error of the mean to show the precision of the estimated population mean, especially if the sample size is small. Ultimately, you should respond respectfully to the reviewers' comments and address any issues or concerns raised in a comprehensive manner.
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