I am using the Cell Biolabs Inc. AAV Purification Standard Kit to purify Adeno-Associated Virus particles, but unfortunately, I have lost the Reagent B. I have checked the product manual and website but cannot find any information about the specific function of this reagent. Does anyone who has used this kit before know what Reagent B is used for and what its composition is? Any help or suggestions would be greatly appreciated.
I’m not familiar with the kit but similar kits use PEG precipitation to bind capsids and pellet them.
If this is what they use, Reagent A would be PEG (white fluffy substance) whereas Reagent B would be NaCl (probably 1 molar)
If Reagent A looks like a whiteish substance, you could probably assume Reagent B is 1M NaCl.
John Counsell
Thank you for your answer. I have attached the protocol of my kit to show where Reagent A and B is used. Does it look similar to yoour PEG/NaCl protocol. Also can you suggest any protocol to purify AVV2?
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