When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View

Checking for overfitting in niche models?

In niche models, overfitting has been traditionally assessed by large difference between training and test AUC or other model performance metrics. Are there other methods to assess model...

07 October 2020 9,060 3 View

How do we balance the argument on the effect of hydroxychloroquine in Covid-19 prophylaxis and treatment?

Hydroxyl-chloroquine has been listed as a potential drug in the management of Covid-19. The effect of this drug in Covid-19 prophylaxis and management is trending on social media. The Doctors...

29 July 2020 2,552 4 View

Positive value in NCT real-time PCR?

Hi I will be happy for your help. I'm doing real-time PCR with two primers called MT-TL1 and 18S, for mtDNA and houskeeping gene, and I got positive control on both NCT. I tried also different...

07 March 2020 3,953 4 View

Can anyone help with a detailed assay procedure for the spectrophotometric measurement of protein carbonyls in tissues?

Protein carbonyls are important compounds to measure progression of oxidative stress in tissues. Available articles online do not have details about how to do final estimation/calculation of the...

06 February 2020 2,897 8 View

What significance is the following?

What is the significance of the following solutions being used as rodent"s organ fixatives, between normal saline, citrate buffer and sucrose?

09 July 2019 5,580 1 View

Suction pump of the Rotary evaporator?

Can someone explain why the suction pump of the rotary evaporator sucks solvent from the condensation chamber, also pumping out engine oil when it's being switched off and perhaps the solution to...

19 June 2019 3,530 13 View

Where can i purchase tail cuff device?

Anyone with the idea of where to buy tail cuff device for measuring the blood pressure of rats. Appreciation in anticipation. Fasan

10 April 2019 2,700 2 View

Defat before phenolic rich fraction extraction?

06 April 2019 2,866 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Can anyone help to find any research articles related to the below mentioned objective?

Astaxanthin used in surimi based products as colour enhancer or as an antioxidant! Regards & Thanks Asik Ikbal

25 January 2021 9,399 4 View

How could i explain the EC50 data utilized by graph Pad prism 9 ?

I measured the FARP antioxidant activity of plant extract than i used graph pad prism 9 to calculate the EC50, now i am not sure how to describe these data as i got the logECF and ECF .

17 January 2021 4,576 3 View

How do i prepare rat colon samples for ANTIOXIDANT assay?

I stored small pieces of rat colon in PBS at -20 and now I want to do SOD and MDA assay, I also need to do the Bradford assay to quantify the protein content in the samples. can anyone tell me...

07 January 2021 2,488 1 View

Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

03 December 2020 6,488 2 View

Lyophilization problem related to Bacteria derived Gold Nanoparticles?

Respected Dr Yuri and Rawle We have prepared the bacteria derived gold nanoparticles and for its antibacterial and antioxidant applications we want exact concentration and for that purpose we...

25 November 2020 2,565 3 View

Anyone familiar with antioxidant studies on rats' brain homogenate?

I am looking for a skilled personnel in Kuwait University that is familiar with antioxidant studies using ELISA kit or other techniques on rat's brain homogenate, particularly the hippocampal and...

04 November 2020 5,481 1 View

What media can be used for real-time H2O2 measurement (intracelular)?

What media or salt solution (HBSS, EBSS, Tyrode) can be used for real-time intracelular h2o2 imaging? I am using a genetically-encoded biosensor based on roGFP and have to find the media that...

28 October 2020 7,301 2 View

How to store cell lysate for MDA assay?

Hi, I am working on HK-2 cells and want to check for MDA accumulation after treatment with our compounds of interest. I am using MDA assay kit from Sigma. However, the kit does not mention about...

03 September 2020 1,795 2 View

Why is it necessary to vortex and incubate in dark the sample and DPPH solution and other DPPH questions?

Hi all, In the determination of Antioxidant capacity of fruit samples, I used DPPH assay and have a few questions regarding the DPPH assay 1) why is it necessary to vortex and incubate in dark...

30 August 2020 246 3 View