What do you think is the best genetic marker to define species?

More Halley K. C. Soares's questions See All

What's the right time to develop/finish mastering and doctorship research and what do you think about the pressure imposed?

31 December 2019 9,243 17 View

How do you do to design an assay with primers and probes that function well and what program/plataform do you use?

How do you do to design an assay with primers and probes that function well and what program/platform do you use? I'm using AlleleID now but I wanna try any other ways to design it.

11 December 2019 5,418 4 View

What do you think about contamination in environmental DNA results?

When working with eDNA we sometimes face some contamination, and it may lead to false-positive or negative, and I think if some of these cases, it creates a problem, or it may be interpreted as a...

01 January 1970 7,858 6 View

Why did we got so smart and the other animals including that ones that we know as our ancestral? Do you think that we are the only intelligent planet?

Well, I think that animals and us (animals too) are both intelligent and rational, we just evolved, back in time we were "dumb" as we think animals are but who thinks this way is wrong, you don't...

01 January 1970 282 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Do you think can be any diamond in A type eclogites?

I want to know more about diamond ore deposits in world.

08 August 2024 1,514 0 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View