A team in China have started an effort to develop CRISPR/Cas9 edited embryos. What is your scientific or ethical opinions?
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We have Melles Griot beam expander and there is exist some dust on inside of laser collimator.
05 June 2014 3,802 6 View
k=-19.252747 slope=-19.252747 -->-19.252747--19.252747 ans = 0. -->k-slope ans = 2.132D-14 So, what is the difference? Note: k and slope calculated...
02 March 2014 2,444 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...
29 July 2024 3,684 0 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Liu Zhaosheng. 2003. Triassic and Jurassic sporopollen assemblage from the Kuqa Depression,Tarim Basin of Xinjiang,NW China. Palaeontologia Sinica, New Series A,no. 14(Whole no. 190)....
28 July 2024 6,390 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View